MILK (CASEIN) ELISA Kit - 96 wells

The BioFront Technologies Milk (casein) ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative or qualitative detection of bovine casein. This monoclonal antibody (MAb)-based assay provides a highly sensitive and specific method for the quantification of bovine casein presence/contamination within a variety of food matrices. Casein is a major milk allergen and was selected based on its strong resistance to food processing and high abundance in bovine milk and a wide variety of milk products. This kit does not detect beta-lactoglobulin, another allergen protein, which represents 20% of total milk protein. Beta-lactoglobulin is enriched to to 60% - 90% in whey and its purified derivitaves. Although most whey preparations contain trace amounts of residual casein, products made with or containing whey protein (rather than whole milk or purified casein) may contain much lower amounts of casein and may therefore not be detected by the casein ELISA kit.

MONOTRACE MILK (CASEIN) ASSAY BACKGROUND

The BioFront Technologies Milk (casein) ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative or qualitative detection of casein in food products. This monoclonal antibody (MAb)-based assay provides a highly sensitive and specific method for the quantification of casein presence/contamination within a variety of food matrices. Casein is a major milk allergen and was selected based on its strong resistance to food processing and high abundance in bovine milk and a wide variety of milk products. This kit does not detect beta-lactoglobulin, another allergen protein, which represents 20% of total milk protein. Beta-lactoglobulin is enriched to to 60% - 90% in whey and its purified derivitaves. Although most whey preparations contain trace amounts of residual casein, products made with or containing whey protein (rather than whole milk or purified casein) may contain much lower amounts of casein and may therefore not be detected by the casein ELISA kit.

MONOTRACE MILK (CASEIN) ASSAY PERFORMANCE

Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation

Number of Tests per Kit: 48

Limit of Detection (casein): 0.11 ppm

Range of Quantification (casein): 0.5 to 8 ppm

Specificity: Bovine casein

THE MONOTRACE ADVANTAGE

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Milk (Casein) ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

• Target a highly stable Milk allergen (casein) resistant to food processing effects
• Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
• Perform well in a wide variety of food products with negligible food matrix interference

CROSS-REACTIVITY

At concentrations as high as 500,000 ppm, no cross-reactivity was observed in a large
panel of assayed samples, including: tree nuts, legumes, seeds, flours (wheat, corn, oat, rice), egg, beef, fish, and crustaceans.

RECOVERY

Recovery of casein-spiked samples was acceptable, according to current AOAC guidelines (J. AOAC Int. 2012), when the following food matrices were assayed: cake mix, corn flour, rice flour, infant soy formula and non-dairy chocolate.
For more details on assay parameters, a full validation report is available upon request.

THE MONOTRACE ADVANTAGE

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Milk (Casein) ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

• Target a highly stable and abundant Milk allergen (casein) resistant to food processing effects
• Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
• Perform well in a wide variety of food products with negligible food matrix interference

CROSS-REACTIVITY

At concentrations as high as 500,000 ppm, no cross-reactivity was observed in a large
panel of assayed samples, including: tree nuts, legumes, seeds, flours (wheat, corn, oat, rice), egg, beef, fish, and crustaceans.

RECOVERY

Recovery of casein-spiked samples was acceptable, according to current AOAC guidelines (J. AOAC Int. 2012), when the following food matrices were assayed: cake mix, corn flour, rice flour, infant soy formula and non-dairy chocolate.
For more details on assay parameters, a full validation report is available upon request.

The BioFront Technologies Milk (casein) ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative or qualitative detection of casein in food products. This monoclonal antibody (MAb)-based assay provides a highly sensitive and specific method for the quantification of casein presence/contamination in a variety of food matrices. Casein is a major milk allergen and was selected based on its strong resistance to food processing and high abundance in bovine milk (82%) and a wide variety of milk-derived/containing products. This kit does not detect beta-lactoglobulin, another milk allergen, which represents 20% of total milk protein. Beta-lactoglobulin is enriched to in whey and its purified derivitaves. Although most whey preparations contain trace amounts of residual casein, products made with or containing primarily whey protein (rather than whole milk or purified casein) may have much lower amounts of casein and may, therefore, not be detected by the casein ELISA kit.