Anti-NS5A (JFH-1) Monoclonal Clone 2F6



ITEM # HCV-2F6

PRICE: $435.00

QTY *  

Clone: 2F6

Volume: 100 ul (0.5 ug/ul)

Specificity: Recognizes domain I of JFH-1 NS5A (genotype 2a)

Form: Protein G purified

Origin: Mouse

Isotype: IgG2a

Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)

Storage: Short Term (oC

Long Term (up to 1 year), -20 C (avoid repeated freeze/thaw)

Applications: Ideal for immunofluorescence. Can also be used for Western Blot at low dilutions (see below).

Working Dilutions: IF-1, 2,000-1:6,000; Western Blot, 1:500

Reference: Backes et al. J Virol. 2010 Jun;84(11):5775-89

Figures

Fig. 1.

Cell lysate run on 12% SDS-PAGE under non-reducing

conditions and transferred to PVDF.

Primary antibody incubation performed overnight at 4oC.

Fig. 2.