Anti-NS5A (JFH-1) Monoclonal Clone 2F6
ITEM # HCV-2F6
PRICE: $435.00
PRICE: $435.00
Clone: 2F6
Volume: 100 ul (0.5 ug/ul)
Specificity: Recognizes domain I of JFH-1 NS5A (genotype 2a)
Form: Protein G purified
Origin: Mouse
Isotype: IgG2a
Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)
Storage: Short Term (oC
Long Term (up to 1 year), -20 C (avoid repeated freeze/thaw)
Applications: Ideal for immunofluorescence. Can also be used for Western Blot at low dilutions (see below).
Working Dilutions: IF-1, 2,000-1:6,000; Western Blot, 1:500
Reference: Backes et al. J Virol. 2010 Jun;84(11):5775-89
Figures
Fig. 1.
Cell lysate run on 12% SDS-PAGE under non-reducing
conditions and transferred to PVDF.
Primary antibody incubation performed overnight at 4oC.
Fig. 2.