Anti-NS5A (JFH-1) Monoclonal Clone 7B5



ITEM # HCV-7B5

PRICE: $435.00

QTY *  

Clone: 7B5

Volume: 100 ul (0.5 ug/ul)

Specificity: Recognizes domain I of JFH-1 NS5A (genotype 2a)

Form: Protein G purified

Origin: Mouse

Isotype: IgG2b

Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)

Storage: Short Term (oC

Long Term (up to 1 year), -20oC (avoid repeated freeze/thaw)

Applications: Western Blot

Working Dilutions: Western Blot, 1:1000-1:10,000

Figure


Cell lysate run on 12% SDS-PAGE and transferred to PVDF.

Primary antibody incubation performed for 1-hour at room temperature

(overnight incubations also suitable).

REFERENCES:


Fridell RA, Valera L, Qiu D, Kirk MJ, Wang C, Gao M. Intragenic complementation of hepatitis C virus NS5A RNA replication-defective alleles. J Virol. 2013 Feb;87(4):2320-9.

Friesland M, Mingorance L, Chung J, Chisari FV, Gastaminza P. Sigma-1 receptor regulates early steps of viral RNA replication at the onset of hepatitis C virus infection. J Virol. 2013 Jun;87(11):6377-90.

Wu X, Robotham JM, Lee E, Dalton S, Kneteman NM, Gilbert DM, Tang H. Productive Hepatitis C Virus Infection of Stem Cell-Derived Hepatocytes Reveals a Critical Transition to Viral Permissiveness during Differentiation. PLoS Pathog 2012; 8 (4).

Gris H, Frausto S, Logan T, Tang H. A conserved tandem cyclophilin-binding site in hepatitis C virus nonstructural protein 5A regulates Alisporivir susceptibility. J Virol. 2012 May;86(9):4811-22.