Anti-NS5B (JFH-1) Monoclonal Clone 4B8



ITEM # HCV-4B8

PRICE: $435.00

QTY *  

Clone: 4B8

Volume: 100 ul (1 ug/ul)

Specificity: Recognizes JFH-1 NS5B (genotype 2a)

Form: Protein G-purified

Origin: Mouse

Isotype: IgG2a

Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)

Storage: Short Term (oC

Long Term (up to 1 year), -20oC (avoid repeated freeze/thaw)

Applications: Western Blot

Working Dilutions: Western Blot, 1:1000-1:5,000


Cell lysate run on 12% SDS-PAGE and transferred to PVDF.

Primary antibody incubation for 1 hour at room temperature

(overnight incubations also suitable at 4oC).

REFERENCES

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Horwitz JA, Dorner M, Friling T, Donovan BM, Vogt A, Loureiro J, Oh T, Rice CM, Ploss A. Expression of heterologous proteins flanked by NS3-4A cleavage sites within the hepatitis C virus polyprotein. Virology. 2013 Apr 25;439(1):23-33.

Nag A, Robotham JM, Tang H. Suppression of Viral RNA Binding and the Assembly of Infectious Hepatitis C Virus Particles In Vitro by Cyclophilin Inhibitors. J Virol. 2012 Dec;86(23):12616-24.

Ferraris P, Beaumont E, Uzbekov R, Brand D, Gaillard J, Blanchard E, Roingeard P. Sequential biogenesis of host cell membrane rearrangements induced by hepatitis C virus infection. Cell Mol Life Sci. 2013 Apr;70(7):1297-306.

Conrad KD, Giering F, Erfurth C, Neumann A, Fehr C, Meister G, Niepmann M. MicroRNA-122 dependent binding of Ago2 protein to hepatitis C virus RNA is associated with enhanced RNA stability and translation stimulation. PLoS One. 2013;8(2):e56272.

Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H. Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation. Nucleic Acids Res. 2009 Oct;37(19):6587-99.