Anti-FLAG Monoclonal Antibody (clone FG4R) 100 ul
PRICE: $195.00
Volume
100 ul (1 ug/ul)
Description
Anti-DYKDDDDK (FLAG) Tag (FG4R) monoclonal antibody
Clonality | Host Species | Isotype
FG4R | Mouse | IgG1
Immunogen
DYKDDDDK (FLAG) synthetic peptide conjugated to KLH
Specificity
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Purification
Protein A-purified from mouse ascites fluid
Application
Dot, ELISA, IP, IS, WB
Sensitivity
WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins
Working Condition
WB (with ECL), 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS, 1:500-2000 dilution
For best results with other assays (e.g., Dot, ELISA, IP, etc.), determine optimal working dilution by titration test
Storage
Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks.
Image
1:1000 (1 g/mL) Ab dilution probed against
HEK293 cells transfected with DYKDDDDK-tagged protein vector;
untransfected (1) and transfected (2)