Anti-GFP Monoclonal Antibody (clone GF28R) 500 ul

Volume

500 ul (1 ug/ul)

Description

Anti-GFP (Green Fluorescent Protein) Tag (GF28R) monoclonal antibody

Clonality | Host Species | Isotype

GF28R | Mouse | IgG2b

Immunogen

GFP from the jellyfish, Aequorea victoria, N-terminal peptide-KLH conjugate

Specificity

Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Purification

Protein A-purified from mouse ascites fluid

Application

Dot, ELISA, IP, IS, WB

Sensitivity

WB (with ECL), 1-10 ng of purified GFP or GFP fusion proteins

Working Condition

WB (with ECL), 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS, 1:500-2000 dilution

For best results with other assays (e.g., Dot, ELISA, IP, etc.), determine optimal working dilution by titration test.

Storage

Store at -20^oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4^oC (add 0.05% NaN₃) for several days to weeks.

Image

1:1000 (1 g/mL) Ab dilution probed against HEK293 cells

transfected with GFP-tagged protein vector:

untransfected control (1), 1 g (2) and 10 g (3) of cell lysates