Anti-HA Monoclonal Antibody (clone HA.C5) 500 ul
PRICE: $435.00
Volume
500 ul (1 ug/ul)
Description
Anti-HA Tag (HA.C5) Monoclonal Antibody
Clonality | Host Species | Isotype
HA.C5 | Mouse | IgG3
Immunogen
YPYDVPDYA (HA) synthetic peptide conjugated to KLH
Specificity
Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins
Purification
Protein A affinity chromatography from mouse ascites fluid
Application
Dot. ELISA, IP, IS, WB
Sensitivity
WB (with ECL): 1-5ng of HA-tagged fusion proteins
Working Condition
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g., Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage
Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks
Image
Fig.
1 2
1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)
- Item #: HA.C5-500