Anti-HA Monoclonal Antibody (clone HA.C5) 500 ul



ITEM # HAC5-500

PRICE: $435.00

QTY *  

Volume

500 ul (1 ug/ul)

Description

Anti-HA Tag (HA.C5) Monoclonal Antibody

Clonality | Host Species | Isotype

HA.C5 | Mouse | IgG3

Immunogen

YPYDVPDYA (HA) synthetic peptide conjugated to KLH

Specificity

Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins

Purification

Protein A affinity chromatography from mouse ascites fluid

Application

Dot. ELISA, IP, IS, WB

Sensitivity

WB (with ECL): 1-5ng of HA-tagged fusion proteins

Working Condition

WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS: 1:500-2000 dilution

For best results with other assays (e.g., Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Storage

Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks

Image


Fig.

1 2

1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2)

  • Item #: HA.C5-500



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