BioID antibody: Chicken Polyclonal Ab to E. coli Biotin Ligase/BirA



ITEM # BID-CP-100

PRICE: $495.00

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Form: Purified Immune IgY from chicken eggs

Immunogen: Recombinant BioID, E. coli BirA R118G, MW 37 kDa

Specificity: Reacts with native E. coli biotin ligase/BirA and the BirA R118G mutant used in BioID studies

Origin: Chicken

Buffer/Composition: 0.01% sodium azide, glycerol (50% v/v), PBS (50% v/v)

Volume: 100 microliters (ul)

Concentration: 10 mg/ml

Storage:

Short Term (<6 months): 2-6oC

Long Term (>6 months to 1 year): -20oC (avoid repeated freeze/thaw)

Working Dilutions:

Western Blot: 1:40,000 (see product brochure)

Immunofluorescence: 1:5,000 (see product brochure)

Shipping: Shipped on ice packs

References:

BioID: Kim DI, Jensen SC, Roux KJ. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.

The Nuclear Envelope. Methods in Molec. Biol. 1411:133-46, 2016

BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012

BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016

Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biol. 26:804–17, 2016

26:804–817



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