MCS-13xLinker-BioID2-HA-pcDNA3.1



ITEM # 13X-BIOID2-HA-PCDNA31

PRICE: $335.00

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Description: MCS-13xLinker-BioID2-HA-pcDNA3.1

Item #: 13X-BioID2-HA-pcDNA3.1

Format: liquid; TAE buffer, pH 8.3

Concentration: 200 ng/ul

Volume: 50 ul (10 ug)

Shipping: shipped on ice

VECTOR INFORMATION

Vector: pCDNDA3.1

Size (bp): 6335

Type: Mammalian Expression

Promoter: CMV

Selectable markers: Neomycin (G418 selectable)

Tags: C-terminal HA tag

Fusions: C-terminal humanized A. aeolicus BioID2 with R40G

FOR TRANSFORMATION

Resistance: Amp

Growth Temp: 37oC

Growth Strain: DH5

REFERENCES

BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016

Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biol. 26:804–17, 2016




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