mycBioID-pBabe-puro
PRICE: $335.00
Description: mycBioID-pBabe-puro
Item #: mycBioID
Format: liquid; TAE buffer, pH 8.3
Concentration: 200 ng/ul
Volume: 50 ul (10 ug)
Shipping: shipped on ice
VECTOR INFORMATION
Vector: pBABEpuro retroviral vector
Size (bp): 6213
Type: Mammalian Expression
Promoter: Viral 5'LTR
Selectable markers: Puromyocin
Tags: N-terminal Myc tag
Fusions: C-terminal humanized E. coli biotin ligase/BirA with R118G
FOR TRANSFORMATION
Resistance: Amp
Growth Temp: 37oC
Growth Strain: DH5
REFERENCES
BioID: Kim DI, Jensen SC, Roux KJ. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.
The Nuclear Envelope. Methods in Molec. Biol. 1411:133-46, 2016
BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012
BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016
Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biol. 26:804–17, 2016