mycBioID-pBabe-puro



ITEM # MYCBIOID

PRICE: $335.00

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Description: mycBioID-pBabe-puro

Item #: mycBioID

Format: liquid; TAE buffer, pH 8.3

Concentration: 200 ng/ul

Volume: 50 ul (10 ug)

Shipping: shipped on ice

VECTOR INFORMATION

Vector: pBABEpuro retroviral vector

Size (bp): 6213

Type: Mammalian Expression

Promoter: Viral 5'LTR

Selectable markers: Puromyocin

Tags: N-terminal Myc tag

Fusions: C-terminal humanized E. coli biotin ligase/BirA with R118G

FOR TRANSFORMATION

Resistance: Amp

Growth Temp: 37oC

Growth Strain: DH5

REFERENCES

BioID: Kim DI, Jensen SC, Roux KJ. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.

The Nuclear Envelope. Methods in Molec. Biol. 1411:133-46, 2016

BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012

BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016

Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biol. 26:804–17, 2016




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