Anti-GFP Monoclonal Antibody (clone GF28R) 1 ml



ITEM # GF28R-1

PRICE: $755.00

QTY *  

Volume

1 ml (1 mg/ml)

Description

Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody

Clonality | Host Species | Isotype

GF28R | Mouse | IgG2b

Immunogen

GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates

Specificity

Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP

Purification

Protein A affinity chromatography from mouse ascites fluid

Application

Dot, ELISA, IP, IS, WB

Sensitivity

WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins

Working Condition

WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS: 1:500-2000 dilution

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Storage

Store at -20 C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 C (add 0.05% NaN3) for several days to weeks

Images

Fig. 1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 g (2) and 10 g (3) of cell lysates used

  • Item #: GF28R-1