Anti-HA Monoclonal Antibody (clone HA.C5) 1 ml



ITEM # HAC5-1

PRICE: $755.00

QTY *  

Volume

1 ml (1 mg/ml)

Description

Anti-HA Tag (HA.C5) Monoclonal Antibody

Clonality | Host Species | Isotype

HA.C5 | Mouse | IgG3

Immunogen

YPYDVPDYA (HA) synthetic peptide conjugated to KLH

Specificity

Recognizes the N-terminal, C-terminal or internal HA- tagged fusion proteins

Purification

Protein A affinity chromatography from mouse ascites fluid

Application

Dot. ELISA, IP, IS, WB

Sensitivity

WB (with ECL): 1-5 ng of HA-tagged fusion proteins

Working Condition

WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS: 1:500-2000 dilution

For best results with other assays (e.g., Dot, ELISA, IP, etc.), please determine optimal working dilution by titration test

Storage

Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks

Image

160311130248_anti-ha.png

1:1000 (1 ug/mL) Ab dilution probed against HEK293 cells

transfected with HA-tagged protein vector;

untransfected (1) and transfected (2)