Anti-His Monoclonal Antibody (clone HIS.H8) 1 ml



ITEM # HISH8-1

PRICE: $600.00

QTY *  

Volume

(1 mg/ml)

Clonality | Host Species | Isotype

HIS.H8 | Mouse | IgG2b

Immunogen

HHHHHH (6x His) synthetic peptide conjugated to KLH

Specificity

Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location within the fusion protein sequences (i.e., reacts with N-terminal, C-terminal or internal 6x His-tags).

Purification

Protein A-purified from mouse ascites fluid

Applications

Dot, ELISA, IP, IS, WB

Sensitivity

WB (with ECL), 1-2 ng of His-tagged fusion protein

Working Conditions

WB (with ECL), 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS, 1:500-2000 dilution

For best results with other assays (e.g., Dot, ELISA, IP, etc.), determine optimal working dilution by titration test

Storage

Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks.

Images


Fig. 1

LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)

Fig. 2

Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 g/mL).


Fig. 3

Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).