Anti-RFP Monoclonal Antibody (clone RF5R) 500 ul

Volume

500 ul (1 ug/ul)

Description

Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody

Clonality | Host Species | Isotype

RF5R | Mouse | IgG1

Immunogen

RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Specificity

Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry,

Purification

Protein A affinity chromatography from mouse ascites fluid

Application

Dot, ELISA, IP, IS, WB

Sensitivity

WB (with ECL): 1-10ng of purified RFP or RFP fusion proteins

Working Condition

WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS: 1:500-2000 dilution

In order to obtain best results in other assays (e.g., Dot, ELISA, IP, etc.), please determine optimal working dilution by titration test

Storage

Store at -20^oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4^oC (add 0.05% NaN₃) for several days to weeks

Images

1 2 3

Fig. 1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with RFP vector; untransfected control (1), transfected with TurboRFP (2), and transfected with dsRed (3)