Lupin ELISA Kit - 96 wells
Lupin (lupine), a legume belonging to the same plant family as peanuts, can serve as a rich source of protein and is being increasingly used in bakery, confectionery, snacks and pastry products world wide due to its multifunctionality. The popularity of lupin as a food ingredient has experienced rapid grow due, in part, to its use as a substitute for gluten-containing flours in gluten-free products. In Europe, where lupin has long been widely used in food products, there has been mandatory allergen labeling for food products containing lupin since 2007 and, in 2016, the Food Standards agency for Australia and New Zealand announced that they were "assessing if lupin should be regulated as food allergen." BioFront's MonoTrace Lupin ELISA kit is best-in-class for lupin detection in foods and beverages and on surfaces.
LUPIN ASSAY PERFORMANCE
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation
Number of Tests per Kit: 96
Limit of Detection: 0.13 ppm
Range of Quantification: 1 to 40 ppm
*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Lupin ELISA incorporates a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable lupin allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
- Perform well on a wide variety of food products with negligible food matrix interference
The assay exhibits strong reactivity to commercially available lupin species (Lupinus angustifolius, Lupinus albus, Lupinus luteus). At concentrations as high as 1,000,000 ppm, no cross-reactivity was observed in a large panel of matrices including other legumes, tree nuts, seeds, flour, meats, and spices.
Recovery of spiked lupin samples was within the acceptable range, according to current AOAC guidelines (J. AOAC Int. 2012), when the following food matrices were assayed: wheat flour, rice flour, oat flour, chickpea flour, lentil flour, chocolate, orange juice, wine, ketchup, crackers, fruit & nut trail mix, assorted baby foods, raw beef & sausage, red onion, steak & taco seasoning, soup mix, breads/cakes/cookies & associated mixes, and ice cream.
Other matrices may also be suitable for assaying with the MonoTrace Lupin ELISA kit. Contact BioFront Technologies technical support (firstname.lastname@example.org) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). An internal validation using a lupin-spiked sample is recommended to ensure that lupin protein is efficiently recovered using the kit.