Buckwheat ELISA Kit - 48 wells

ITEM # BW1-EK-48

PRICE: $395.00

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Assay Time: 30 minutes post-extraction

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 48

Limit of Detection: 0.08 ppm

Range of Quantification: 1 to 40 ppm

Specificity: Buckwheat

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.


BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Buckwheat ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target a highly stable Buckwheat allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
  • Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to buckwheat (Fagopyrum esculentum). At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other seed, nut, legume, or meat matrices tested using the MonoTrace Buckwheat ELISA kit. Minimal cross-reactivity (0.0012-0.0028%) or contamination was observed when certain commercial grains and spices were assayed.


The MonoTrace Buckwheat ELISA kit is suitable for the qualitative or quantitative detection of buckwheat in various food matrices including flours, meats, pastas, and cereals. Recoveries were acceptable according to current AOAC guidelines (J. AOAC Int. 2012). In summary, buckwheat was efficiently extracted and detected using the
MonoTrace Buckwheat ELISA from common matrices including flours, meats, pastas, and cereals.

Other matrices may also be suitable for assaying using the MonoTrace Buckwheat ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). An internal validation by the user with a buckwheat-spiked sample is recommended to ensure that buckwheat is efficiently recovered when using the kit.