Pine Nut ELISA Kit - 96 wells

ITEM # PN1-EK-96

PRICE: $550.00

QTY *  

Pine Nuts (Pignolias) are technically seeds, but are typically classified as tree nuts. BioFront's MonoTrace ELISA kit is one of the few methods available to accurately detect Pine Nuts in foods and on surfaces.


Assay Time: 30 minutes post-extraction

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 96

Limit of Detection: 0.24 ppm

Range of Quantification: 1 to 40 ppm

Specificity: Pine nut

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.


BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Pine Nut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target a highly stable Pine Nut allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
  • Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to pine nut (Pinus pinea). At concentrations as high as 100,000 ppm Pecan (0.0020%), walnut (0.0019%), and buckwheat (0.0012%) were minimally cross-reactive. No cross reactivity was observed within other matrices tested including a large panel of tree nuts, legumes, seeds, flours,meats and spices.


The MonoTrace Pine Nut ELISA kit is suitable for the qualitative or quantitative detection of pine nut in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, pine nut protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Pine Nut ELISA.

Other matrices may also be suitable for use with the MonoTrace Pine Nut ELISA kit. Contact BioFront Technologies technical support ( for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). A user internal validation using a pine nut-spiked sample is recommended to ensure that pine nut is efficiently recovered before qualitative or quantitative results are obtained from the kit.