Anti-FLAG Monoclonal Antibody (clone FG4R) 100 ul



ITEM # FLAGFG4R-100

PRICE: $195.00

QTY *  

Volume

100 ul (1 ug/ul)

Description

Anti-DYKDDDDK (FLAG) Tag (FG4R) monoclonal antibody

Clonality | Host Species | Isotype

FG4R | Mouse | IgG1

Immunogen

DYKDDDDK (FLAG) synthetic peptide conjugated to KLH

Specificity

Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Purification

Protein A-purified from mouse ascites fluid

Application

Dot, ELISA, IP, IS, WB

Sensitivity

WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins

Working Condition

WB (with ECL), 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS, 1:500-2000 dilution

For best results with other assays (e.g., Dot, ELISA, IP, etc.), determine optimal working dilution by titration test

Storage

Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks.

Image


1:1000 (1 g/mL) Ab dilution probed against

HEK293 cells transfected with DYKDDDDK-tagged protein vector;

untransfected (1) and transfected (2)