Gluten ELISA Kit - 48 wells
MONOTRACE GLUTEN ELISA KIT
Monoclonal antibodies: The MonoTrace Gluten ELISA is the first assay to incorporate the use of two new gliadin-specific monoclonal antibodies, clones 13F6 and 14G11.
Extraction: For use in detecting and accurately quantifying gluten contamination in raw food ingredients and processed foods. Extraction (1 hour at 60oC) from a variety of food utilizes a new non-toxic extraction buffer, the MonoTrace Gluten Extraction Buffer (MGEB).
Recovery: Recovery from various food matrices and ingredients (n=18) was analyzed using in-house incurred cornbread samples, gluten-free (GF) commercial products and ingredients, as wells as quality control and reference materials sourced from the Food Analysis Performance Assessment Scheme (FAPAS) division of the Food and Environment Research Agency (FERA). Recovery of gluten from in-house gluten-incurred cornbread and gluten-spiked commercial food matrices was >75%.
Cross-reactivity: Cross-reactivity was tested using a large panel of GF and non-GF-labeled commercial samples from various vendors (n=126). No cross-reactivity was observed in any of the tree nuts, legumes, seeds, meats or spice samples tested. Wheat, rye and barley flours were highly cross-reactive. The assay exhibits little to no cross-reactivity to several oat cultivars (n=5).
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for sample incubation, conjugate incubation, and substrate incubation.
Number of Tests per Kit: 48
Limit of Detection: 0.36 ppm gluten (0.18 ppm gliadin)
Range of Quantification: 2-100 ppm gluten (1-50 ppm gliadin)
WHY CHOOSE THE MONOTRACE GLUTEN ELISA?
- No hazardous waste generated!
- Results in under 2 hours!
- Sample kits and supporting quality control/reference materials available to help with internal validations. CLICK TO REQUEST FREE TRIAL!