Peanut ELISA Kit - 48 wells
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation
Number of Tests per Kit: 48
Limit of Detection: 0.24 ppm
Range of Quantification: 1 to 40 ppm
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Peanut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Peanut allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
The assay exhibits strong reactivity to peanut (Arachis hypogaea). Nutmeg (0.0026%), cayenne pepper (0.0011%), and pumpkin seed (0.0025%) were minimally reactive in the assay. Given the reported adulteration of some commercial spices with peanut in recent years, it is possible that the reactivity observed in certain matrices results from similar circumstances and does not represent cross-reactivity within the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed when testing a variety of other matrices, including tree nuts, legumes, seeds and flours.
The MonoTrace Peanut ELISA kit is suitable for the qualitative or quantitative detection of peanut in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, peanut protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Peanut ELISA.
Other matrices may also be suitable for use with the MonoTrace Peanut ELISA kit. Contact BioFront Technologies technical support (email@example.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). A user internal validation using a peanut-spiked sample is recommended to ensure that peanut is efficiently recovered before qualitative or quantitative results are obtained from the kit.