Mustard ELISA Kit - 96 wells
MUSTARD ASSAY PERFORMANCE
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.
Number of Tests per Kit: 96
Limit of Detection: 0.13 ppm
Range of Quantification: 1 to 40 ppm
Specificity: Mustard seed
*= calculations of parameters are based on representative data from multiple assays using 10-minute incubation steps at room temperature (20-23.5 C / 68-74.3 F). Higher temperatures may result in elevated absorbance readings for samples and standards.
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Mustard ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Mustard allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
At concentrations as high as 100,000 ppm, no cross-reactivity was observed in a large panel of assayed samples, including tree nuts, legumes, grains and cereals.
Varying levels of cross-reactivity was observed within members of the Brassica family: yellow (100%), brown (121%) and black (134%) mustard seeds, cabbage seed (121%), rapeseed (119%), and radish seed (107%). Pinto (0.0014%) and romano (0.0018%) beans, amaranth (0.0018%), quinoa (0.0013%) and rye (0.0007%) flours as well as cress (0.0029%) were minimally cross-reactive in the assay.
Recovery of mustard spiked at various concentrations was within acceptable limits, according to current AOAC guidelines, from common food matrices such as soups, salad dressings, condiments, cheeses, meats and spices.