Sesame ELISA Kit - 48 wells
SESAME ASSAY PERFORMANCE
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.
Number of Tests per Kit: 48
Limit of Detection: 0.3 ppm
Range of Quantification: 1 to 40 ppm
*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Sesame ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Sesame allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
At concentrations as high as 100,000 ppm, no cross-reactivity was observed in a large panel of assayed samples, including walnut, hazelnut, pecan, peanut, pistachio, cashew, pecan, macadamia nut, Brazil nut, almond, and pine nut.
Recovery of spiked almond samples at various concentrations was efficient (>90%) when the following food matrices were assayed: chocolate, cereals, ice cream, cake, and cookies.