Cashew ELISA Kit - 96 wells
Cashews are sourced from Africa, Asia and South America. In the US, Cashew nut allergy is the second most commonly reported Tree nut allergy. Unlike other ELISA methods, BioFront's MonoTrace ELISA kit is highly specific to Cashew.
Assay Time: Now just 30 minutes post-extraction!
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.
Number of Tests per Kit: 96
Limit of Detection: 0.12 ppm
Range of Quantification: 1 to 40 ppm
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Cashew ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target native Cashew allergen as well as allergen in processed foods
- Recognize a defined protein specific target, selected to eliminate cross-reactivity, reducing the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
The assay exhibits strong reactivity to cashew (Anacardium occidentale). At concentrations as high as 100,000 ppm, walnut (0.13%), pecan (0.05%), and pistachio (0.01%) were minimally cross-reactive in the assay. No cross-reactivity was observed with the other matrices including a broad range of tree nuts, legumes, seeds, flours, meats and spices.
The MonoTrace Cashew ELISA kit is suitable for the qualitative or quantitative detection of cashew in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, cashew protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Cashew ELISA.
Other matrices may also be suitable for use with the MonoTrace Cashew ELISA kit. Contact BioFront Technologies technical support (email@example.com) for assistance to determine whether your matrix-of-interest has been validated (cross reactivity & recovery). A user internal validation using a cashew-spiked sample is recommended to ensure that cashew is efficiently recovered before qualitative or quantitative results are obtained from the kit.