Hazelnut ELISA Kit - 48 wells
Allergies to Hazelnut may be due to the seed (nut) or tree pollen. BioFront's MonoTrace ELISA kit has been carefully designed to provide optimum detection nut in a variety of foods and beverages.
Assay Time: 30 minutes post-extraction!
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation
Number of Tests per Kit: 48
Limit of Detection: 0.04 ppm
Range of Quantification: 1 to 40 ppm
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Hazelnut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Hazelnut allergen resistant to food processing effects
- Recognize a carefully selected to eliminate cross-reactivity and thereby reduce the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
The assay exhibits strong reactivity to hazelnut (Corylus Americana) and to a lesser extent walnut (1.08%). Pecan (0.011%) and fava bean (0.0018%) were minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other tested matrices (including tree nuts, legumes, seeds, flours, meats and spices) using the MonoTrace Hazelnut ELISA kit.
The MonoTrace Hazelnut ELISA kit is suitable for the qualitative or quantitative detection of hazelnut in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, hazelnut protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Hazelnut ELISA.
Other matrices may also be suitable for use with the MonoTrace Hazelnut ELISA kit. Contact BioFront Technologies technical support (firstname.lastname@example.org) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). A user internal validation using a hazelnut-spiked sample is recommended to ensure that hazelnut is efficiently recovered before qualitative or quantitative results are obtained from the kit.