Macadamia Nut ELISA Kit - 96 wells
Macadamia nuts are seeds, but are classified as tree nuts for allergen purposes. Allergic individuals have shown cross-reactivity to Hazelnuts, but BioFront's MonoTrace ELISA demonstrates little cross-reactivity between the two.
MACADAMIA NUT ASSAY PERFORMANCE
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation
Number of Tests per Kit: 96
Limit of Detection: 0.13 ppm
Range of Quantification: 2 to 80 ppm
Specificity: Macadamia nut
*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and environmental surfaces. The MonoTrace Macadamia Nut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Macadamia allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
The assay exhibits strong reactivity to macadamia nut (Macadamia integrifolia). When other tree nuts were tested at concentrations as high as 100,000 ppm, only pecan (0.0036%) demonstrated minimal cross-reactivity in the assay while various seeds, meats, and spices showed no cross-reactivity. Other matrices including certain legumes and flours demonstrated minimal cross-reactivity when tested using the MonoTrace Macadamia ELISA Kit.
The MonoTrace Macadamia ELISA kit is suitable for the qualitative or quantitative detection of macadamia in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, macadamia protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Macadamia ELISA.
Other matrices may also be suitable for use with the MonoTrace Macadamia ELISA kit. Contact BioFront Technologies technical support (email@example.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). A user internal validation using a macadamia-spiked sample is recommended to ensure that Macadamia is efficiently recovered before qualitative or quantitative results are obtained from the kit.