Pistachio ELISA Kit - 96 wells
The allergenic proteins associated with Pistachios have only recently been identified. BioFront's MonoTrace ELISA kit is highly specific to Pistachio, unlike other methods, which are prone to cross-react to cashew allergen.
Assay Time: 30 minutes post-extraction
Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.
Number of Tests per Kit: 96
Limit of Detection: 0.12 ppm
Range of Quantification: 1 to 40 ppm
THE MONOTRACE ADVANTAGE
BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Pistachio ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:
- Target a highly stable Pistachio allergen resistant to food processing effects
- Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
- Perform well in a wide variety of food products with negligible food matrix interference
The assay exhibits strong reactivity to pistachio (Pistacia vera). Pecan (0.001%) was minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed in the other matrices tested using the MonoTrace Pecan ELISA kit including tree nuts, legumes, seeds, flour, meats and spices.
The MonoTrace Pistachio ELISA kit is suitable for the qualitative or quantitative detection of pine nut in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, pistachio protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Pistachio ELISA.
Other matrices may also be suitable for use with the MonoTrace Pistachio ELISA kit. Contact BioFront Technologies technical support (email@example.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). A user internal validation using a pistachio nut-spiked sample is recommended to ensure that pistachio is efficiently recovered before qualitative or quantitative results are obtained from the kit.