Walnut ELISA Kit - 96 wells

ITEM # WJ4-EK-96

PRICE: $550.00

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Walnuts are used extensively in foods, especially in baked goods, and are the leading cause of allergic reactions to Tree Nuts in the US. Walnut and Pecan are cross-reactive in many allergic individuals, but, compared to other detection methods, BioFront's MonoTrace ELISA kit shows a higher degree of specificity to Walnut.


Assay Time: 30 minutes post-extraction

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 96

Limit of Detection: 0.22 ppm

Range of Quantification: 1 to 40 ppm

Specificity: Walnut


BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of food allergen contamination in various food samples and on environmental surfaces. The MonoTrace Walnut ELISA incorporates the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

  • Target a highly stable Walnut allergen resistant to food processing effects
  • Recognize a carefully selected target protein to eliminate cross-reactivity and reduce the possibility of 'false positive' results
  • Perform well in a wide variety of food products with negligible food matrix interference


The assay exhibits strong reactivity to walnut (Juglans regia). Pecan (0.0023%), adzuki bean (0.002%), rapeseed (0.0014%),and nutmeg (0.0018%) were minimally reactive in the assay. At concentrations as high as 100,000 ppm, no cross-reactivity was observed within the other matrices tested using the MonoTrace Walnut ELISA kit, including tree nuts, legumes, seeds, flour, meats and spices.


The MonoTrace Walnut ELISA kit is suitable for the qualitative or quantitative detection of pine nut in cake, cookie, chocolate, ice cream and spices. Chocolate, ice cream and spice extractions require the addition of 5% (w/v) non-fat dry milk (NFDM). This extraction additive is needed for samples containing polyphenols, which include: chocolate, fruit, tea, coffee, red wine and soybean and others not listed here. Recoveries were within the acceptable range, as defined by current AOAC guidelines (J. AOAC Int. 2012). In summary, walnut protein was efficiently extracted and detected from common food matrices and spices using the MonoTrace Walnut ELISA.

Other matrices may also be suitable for use with the MonoTrace Walnut ELISA kit. Contact BioFront Technologies technical support (support@biofronttech.com) for assistance to determine whether your matrix-of-interest has been validated (cross-reactivity & recovery). A user internal validation using a walnut-spiked sample is recommended to ensure that walnut is efficiently recovered before qualitative or quantitative results are obtained from the kit.