BioID2 Antibody: Chicken Polyclonal Ab to Aquifex aeolicus biotin ligase for use with BioID2
PRICE: $495.00
Form: Purified Immune IgY from chicken eggs
Specificity: Reacts with native A. aeolicus biotin ligase and the R40G mutant used in BioID2
Buffer/Composition: 0.01% sodium azide, glycerol (50% v/v), PBS (50% v/v)
Volume: 100 microliters (ul)
Concentration: 10 mg/ml
Storage:
Short Term (<6 months): 2-6oC
Long Term (>6 months to 1 year): -20oC (avoid repeated freeze/thaw)
Working Dilutions:
Western Blot, 1:40,000 (see product brochure)
Immunofluorescence, 1:5,000 (see product brochure)
Shipping: Shipped on ice packs
Images:
References:
BioID: Kim DI, Jensen SC, Roux KJ. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.
The Nuclear Envelope. Methods in Molec. Biol. 1411:133-46, 2016
BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012
BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016
Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biol. 26:804–17, 2016
BioID: Kim DI, Jensen SC, Roux KJ. Identifying Protein-Protein Associations at the Nuclear Envelope with BioID.
The Nuclear Envelope. Methods in Molec. Biol. 1411:133-46, 2016
BioID: Roux KJ, Kim DI, Raida M, Burke BJ. A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Cell Biol. 196(6):801-10, 2012
BioID2: Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux KJ. An improved smaller biotin ligase for BioID proximity labeling. Mol Biol Cell. 27(8):1185-96, 2016
Review: Kim DI, Roux KJ. Filling the Void: Proximity-Based Labeling of Proteins in Living Cells. Trends in Cell Biol. 26:804–17, 2016
