Anti-NS3 (JFH-1) Monoclonal Clone 2E3



ITEM # HCV-2E3

PRICE: $435.00

QTY *  

Clone: 2E3

Volume: 100 ul (1 ug/ul)

Specificity: Recognizes the helicase domain of JFH-1 NS3 (genotype 2a)

Cross-reactivity: Cross-reactive against genotype 1a

Form: Protein A-purified

Origin: Mouse

Isotype: IgG1

Buffer/Composition: 1X PBS pH 7.2; 0.1% sodium azide (NaN3)

Storage: Short Term (oC
Long Term (up to 1 year): -20oC (avoid repeated freeze/thaw)

Applications: Western, IF, IP, FACS, and TCID50 assay

Working Dilutions: TCID50, 1:1000-1:5000; Western Blot, 1:1000-1:5000

IF, 1:1000; FACS, 1:500

References:

(1) Fahmy et al. Sci Rep. 2017 Jan 9;7:40351. doi: 10.1038/srep40351.

(2) Chong et al. J Biol Chem. 2016 Feb 19;291(8):3918-31. doi: 10.1074/jbc.M115.675413. Epub 2015 Dec 23.

(3) Pe rez-Berna et al. ACS Nano. 2016 Jul 26;10(7):6597-611.

(4) Saeed et al. Nature. 2015 Aug 27;524(7566):471-5. doi: 10.1038/nature14899. Epub 2015 Aug 12.

(5) Afzal et al. J Gen Virol. 2015 Feb;96(Pt 2):311-21. doi: 10.1099/vir.0.070433-0. Epub 2014 Oct 28.

(5) Brennd rfer et al. J Immunol. 2014 Feb 15;192(4):1671-80. doi: 10.4049/jimmunol.1301077. Epub 2014 Jan 17.

(6) Paul et al. J Virol. 2013 Oct;87(19):10612-27. doi: 10.1128/JVI.01370-13. Epub 2013 Jul 24.

(6) Friesland et al. J Virol. 2013 Jun;87(11):6377-90. doi: 10.1128/JVI.03557-12. Epub 2013 Mar 27.

(7) Fridell et al. J Virol. 2013 Feb;87(4):2320-9.

(8) Wu et al. PLoS Pathog. 2012;8(4):e1002617. doi: 10.1371/journal.ppat.1002617. Epub 2012 Apr 5.

(9) Jirasko et al. PLoS Pathog. 2010 Dec 16;6(12):e1001233.

(10) Backes et al. J Virol. 2010 Jun;84(11):5775-89

(11) Kenworthy et al. Nucleic Acids Res. 2009 Oct;37(19):6587-99.

(12) Kaul et al. PLoS Pathog. 2009 Aug;5(8):e1000546

(13) Yang et al. J Virol. 2008 Jun;82(11):5269-78.

Figures




Fig. 1. Cell lysate run on 12% SDS-PAGE and transferred to PVDF.
Primary antibody incubation performed for 1-hour at room temperature
(overnight incubations also suitable at 4oC).
Fig. 2.

Nucleic Acids Res. 2009 Oct;37(19):6587-99.