Anti-GFP Monoclonal Antibody (clone GF28R) 1 ml
PRICE: $755.00
Volume
1 ml (1 mg/ml)
Description
Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody
Clonality | Host Species | Isotype
GF28R | Mouse | IgG2b
Immunogen
GFP from the jellyfish Aequorea victoria, N-terminal peptide-KLH conjugates
Specificity
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Purification
Protein A affinity chromatography from mouse ascites fluid
Application
Dot, ELISA, IP, IS, WB
Sensitivity
WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
Working Condition
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g., Dot, ELISA, IP, etc.), please determine optimal working dilution by titration test
Storage
Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks
Images
Fig. 1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1 g (2) and 10 g (3) of cell lysates used
- Item #: GF28R-1