Anti-GFP Monoclonal Antibody (clone GF28R) 500 ul
PRICE: $435.00
Volume
500 ul (1 ug/ul)
Description
Anti-GFP (Green Fluorescent Protein) Tag (GF28R) monoclonal antibody
Clonality | Host Species | Isotype
GF28R | Mouse | IgG2b
Immunogen
GFP from the jellyfish, Aequorea victoria, N-terminal peptide-KLH conjugate
Specificity
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Purification
Protein A-purified from mouse ascites fluid
Application
Dot, ELISA, IP, IS, WB
Sensitivity
WB (with ECL), 1-10 ng of purified GFP or GFP fusion proteins
Working Condition
WB (with ECL), 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS, 1:500-2000 dilution
For best results with other assays (e.g., Dot, ELISA, IP, etc.), determine optimal working dilution by titration test.
Storage
Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks.
Image
1:1000 (1 g/mL) Ab dilution probed against HEK293 cells
transfected with GFP-tagged protein vector:
untransfected control (1), 1 g (2) and 10 g (3) of cell lysates