Anti-His Monoclonal Antibody (clone HIS.H8) 500 ul
PRICE: $395.00
Volume
500 ul (1 ug/ul)
Clonality | Host Species | Isotype
HIS.H8 | Mouse | IgG2b
Immunogen
HHHHHH (6x His) synthetic peptide conjugated to KLH
Specificity
Recognizes the 6x His-tag epitope encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e., reacts with N-terminal, C-terminal or internal 6x His-tags).
Purification
Protein A-purified from mouse ascites fluid
Applications
Dot, ELISA, IP, IS, WB
Sensitivity
WB (with ECL): 1-2ng of His-tagged fusion proteins
Working Conditions
WB (with ECL), 1:1000-3000 dilution (incubate for one hour at room temperature)
IS, 1:500-2000 dilution
For best results with other assays (e.g., Dot, ELISA, IP, etc.), please determine optimal working dilution by titration test.
Storage
Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks.
Images
Fig. 1
LEFT: untransfected control; RIGHT: anti-His (red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (blue).
Fig. 2
Comparison between anti-His tag (HIS.H8) mAb with two different vendors' Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5 g/mL).
Fig. 3
Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).
