Anti-Myc Monoclonal Antibody (clone MYC.A7) 1 ml

Volume

1 ul (1 mg/ml)

Description

Anti-Myc Tag (MYC.A7) Monoclonal Antibody

Clonality | Host Species | Isotype

MYC.A7 | Mouse | IgG1

Immunogen

EQKLISEEDL (Myc) synthetic peptide conjugated to KLH

Specificity

Recognizes the N-terminal or C-terminal Myc-tagged fusion proteins

Purification

Protein A affinity chromatography from mouse ascites fluid

Application

Dot, ELISA, IP, IS, WB

Sensitivity

WB (with ECL): 10-50ng of Myc-tagged fusion proteins

Working Condition

WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)

IS: 1:500-2000 dilution

For best results with other assays (e.g., Dot, ELISA, IP, etc.), determine optimal working dilution by titration test

Storage

Store at -20^oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4^oC (add 0.05% NaN₃) for several days to weeks

Images

1 2

Fig. 1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)