Anti-RFP Monoclonal Antibody (clone RF5R) 500 ul
PRICE: $435.00
Volume
500 ul (1 ug/ul)
Description
Anti-RFP (Red Fluorescent Protein) Tag (RF5R) Monoclonal Antibody
Clonality | Host Species | Isotype
RF5R | Mouse | IgG1
Immunogen
RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates
Specificity
Recognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry,
Purification
Protein A affinity chromatography from mouse ascites fluid
Application
Dot, ELISA, IP, IS, WB
Sensitivity
WB (with ECL): 1-10ng of purified RFP or RFP fusion proteins
Working Condition
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
In order to obtain best results in other assays (e.g., Dot, ELISA, IP, etc.), please determine optimal working dilution by titration test
Storage
Store at -20oC for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4oC (add 0.05% NaN3) for several days to weeks
Images
1 2 3
Fig. 1:1000 (1 g/mL) Ab dilution probed against HEK293 cells transfected with RFP vector; untransfected control (1), transfected with TurboRFP (2), and transfected with dsRed (3)
- Item #: RF5R-500