YFV NS1 Mab BF-077-1mg

ITEM # BF-077-1MG

PRICE: $3500.00

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Catalog Number: BF-077-1mg

Immunogen: Cell-culture derived viral lysate from strain Yellow Fever strain 17D

Purification: Purified by anion exchange column chromatography

Isotype: IgG2a

Purity: >90% by SDS-PAGE

Specificity: Yellow Fever virus NS1 protein

Origin: Mouse

Buffer/Composition: 15 mM potassium phosphate, 0.85% NaCl, 0.05% NaN3; pH 7.2

Volume: 1 ml (1 mg)

Concentration: 1 mg/ml


Short Term (less than 6 months): 4oC

Long Term (up to 1 year): -20oC (avoid repeated freeze/thaw)

Working Dilutions:

Western Blot- 1:1000

IF- 1:200-1:1000

ELISA- to be determined by user

Shipping: Shipped on ice packs

Figure 1: Immunoflourescent staining with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-077.

GFP-tagged Yellow Fever-17D virus was used to infect Huh-7.5 (P33) cells for 48 hours. Cells were fixed and stained with BioFront's anti-YFV NS1 clone BF-077 @ 1:200 for 2 hours at RT. Secondary antibody (goat anti-mouse Alexa 594) was incubated for 30 minutes at @ RT. Left panel shows infection as identified by the BF-077 anti-YFV NS1 monoclonal antibody (red) and the right panel shows the overlap with the viral GFP tag.

Figure 2. Western blot with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-077.

Primary antibody was diluted 1:1000 and incubated overnight at 4 C while rocking. Secondary antibody (goat-anti-mouse IgG-HRP from Santa Cruz Biotech) was diluted 1:2000 and incubated 1 hour at RT. (-) = uninfected Huh 7.5 cell lysate; ( ) = YFV-infectedcelllysate collected 48-hours post-infection with Yellow Fevery-17D virus. Arrow points to 45kD band representing YFV NS1 protein.

The above images are property of BioFront Technologies Inc., all rights reserved.