YFV NS1 Mab BF-077-1mg
Catalog Number: BF-077-1mg
Immunogen: Cell-culture derived viral lysate from strain Yellow Fever strain 17D
Purification: Purified by anion exchange column chromatography
Purity: >90% by SDS-PAGE
Specificity: Yellow Fever virus NS1 protein
Buffer/Composition: 15 mM potassium phosphate, 0.85% NaCl, 0.05% NaN3; pH 7.2
Volume: 1 ml (1 mg)
Concentration: 1 mg/ml
Short Term (less than 6 months): 4oC
Long Term (up to 1 year): -20oC (avoid repeated freeze/thaw)
Western Blot- 1:1000
ELISA- to be determined by user
Shipping: Shipped on ice packs
Figure 1: Immunoflourescent staining with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-077.
GFP-tagged Yellow Fever-17D virus was used to infect Huh-7.5 (P33) cells for 48 hours. Cells were fixed and stained with BioFront's anti-YFV NS1 clone BF-077 @ 1:200 for 2 hours at RT. Secondary antibody (goat anti-mouse Alexa 594) was incubated for 30 minutes at @ RT. Left panel shows infection as identified by the BF-077 anti-YFV NS1 monoclonal antibody (red) and the right panel shows the overlap with the viral GFP tag.
Figure 2. Western blot with BioFrontAnti-YFV NS1 protein monoclonal antibody BF-077.
Primary antibody was diluted 1:1000 and incubated overnight at 4 C while rocking. Secondary antibody (goat-anti-mouse IgG-HRP from Santa Cruz Biotech) was diluted 1:2000 and incubated 1 hour at RT. (-) = uninfected Huh 7.5 cell lysate; ( ) = YFV-infectedcelllysate collected 48-hours post-infection with Yellow Fevery-17D virus. Arrow points to 45kD band representing YFV NS1 protein.
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