HRP-labeled Anti-Zika NS1 Mab
Catalog Number: BF-1125-36-HRP
Conjugation: HRP-conjugated monoclonal antibody BF-1125-36
Specificity: Zika virus NS1 protein
Buffer/Composition: Conjugate stabilization buffer
Volume: 50 ul conjugated antibody
Antibody Concentration: 1 mg/ml
Short Term (less than 3 months): 4oC
Long Term (up to 1 year): -20oC (avoid repeated freeze/thaw)
Working Dilutions: Western Blot, 1:1000
Shipping: Shipped on ice packs
Figure 1. Immunoprecipitation Assay using BioFront's Anti-ZIKV NS1 MAbs.
Anti-ZIKV NS1 monoclonal antibodies 1125-06, 1125-36 or normal mouse IgG control were coupled to protein A/G sepharose and subsequently incubated with ZIKV-infected SNB-19 (human brain astrocytes) cell lysate. Lysate input (input), antibody-coupled bead flow thru (flow thru), and bead eluates (eluate) were then run on SDS-PAGE, transferred to PVDF, and probed with an HRP-conjugated anti-ZIKV NS1 monoclonal antibody (BF-1125-36-HRP). The majority of the ZIKV NS1 protein was identified in the flow thru when IP was performed withthe mouse IgG control antibody, while BioFront's anti-ZIKV NS1 monoclonals all successfully preciptated the protein from the lysate.